000			03226cam a2200325 i 4500
001			17962418
003			EG-ScBUE
005			20260124112130.0
008			131204t2014 ne a f b 000 0 eng d
020			_a9789400775442 (alk. paper)
040			_aCDX _beng _erda _cCDX _dYDXCP _dBTCTA _dIXA _dSHH _dDLC _dEG-ScBUE
082	0	4	_222 _a572.877 _bMON
100	1		_aMondal, Partha Pratim, _eauthor. _939564
245	1	0	_aFundamentals of fluorescence microscopy : _bexploring life with light / _cPartha Pratim Mondal, Alberto Diaspro.
264		1	_aDordrecht : _bSpringer, _c[2014]
264		4	_cc2014
300			_axv, 218 pages : _billustrations (some color) ; _c29 cm
336			_2rdacontent _atext _btxt
337			_aunmediated _2rdamedia _bn
338			_avolume _bnc _2rdacarrier
504			_aIncludes bibliographical references.
505	0		_aRay Optics, Wave Optics and Imaging System Design -- Basics of Electromagnetic Theory for Fluorescence Microscopy -- Electric Field Effects in Optical Microscopy Systems -- Quantum Description of Radiation Field and Optical Microscopy -- Molecular Physics of Fluorescent Markers -- Basics of Fluorescence and Photophysics -- General Fluorescence Imaging Techniques -- Multiphoton Fluorescence Microscopy -- Super-Resolution Fluorescence Microscopy -- Image Reconstruction for Fluorescence Microscopy -- Future Prospective of Fluorescence Microscopy.
520			_aThis book starts at an introductory level and leads reader to the most advanced developments in fluorescence imaging and super-resolution techniques that have enabled the emergence of new disciplines such as nanobioimaging, multiphoton microscopy, photodynamic therapy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going deep into the advanced imaging systems and their applications. Molecular orbital theory forms the basis for understanding fluorescent molecules and thereby facilitates complete explanation of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential fluorescence factors and phenomena such as, molecular cross-section, Stokes shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These phenomena form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part covers many imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction limited and unlimited imaging.--
650		7	_aFluorescence microscopy. _2BUEsh _939565
651			_2BUEsh
653			_bENGGEN _cMarch2016
655			_vReading book _934232
700	1		_aDiaspro, Alberto, _d1959- _eauthor.
942			_2ddc _cBB
999			_c21425 _d21397