03226cam a2200325 i 4500 17962418 EG-ScBUE 20260124112130.0 131204t2014 ne a f b 000 0 eng d 9789400775442 (alk. paper) CDX eng rda CDX YDXCP BTCTA IXA SHH DLC EG-ScBUE 22 572.877 MON Mondal, Partha Pratim, author. 39564 Fundamentals of fluorescence microscopy : exploring life with light / Partha Pratim Mondal, Alberto Diaspro. Dordrecht : Springer, [2014] c2014 xv, 218 pages : illustrations (some color) ; 29 cm rdacontent text txt unmediated rdamedia n volume nc rdacarrier Includes bibliographical references. Ray Optics, Wave Optics and Imaging System Design -- Basics of Electromagnetic Theory for Fluorescence Microscopy -- Electric Field Effects in Optical Microscopy Systems -- Quantum Description of Radiation Field and Optical Microscopy -- Molecular Physics of Fluorescent Markers -- Basics of Fluorescence and Photophysics -- General Fluorescence Imaging Techniques -- Multiphoton Fluorescence Microscopy -- Super-Resolution Fluorescence Microscopy -- Image Reconstruction for Fluorescence Microscopy -- Future Prospective of Fluorescence Microscopy. This book starts at an introductory level and leads reader to the most advanced developments in fluorescence imaging and super-resolution techniques that have enabled the emergence of new disciplines such as nanobioimaging, multiphoton microscopy, photodynamic therapy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going deep into the advanced imaging systems and their applications. Molecular orbital theory forms the basis for understanding fluorescent molecules and thereby facilitates complete explanation of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential fluorescence factors and phenomena such as, molecular cross-section, Stokes shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These phenomena form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part covers many imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction limited and unlimited imaging.-- Fluorescence microscopy. BUEsh 39565 BUEsh ENGGEN March2016 Reading book 34232 Diaspro, Alberto, 1959- author. ddc BB 21425 21397 0 0 ddc 0 0 Baccah MAIN MAIN 1ST 2016-03-15 Purchase 240.00 1 6 572.877 MON 000032054 2025-07-15 00:00:00 2016-03-20 BB